Modified QuEChERS combined with UPLC-MS/MS to determine eight biogenic amines in Xinjiang smoked horsemeat sausages

A modified QuEChERS method coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for the simultaneous determination of eight biogenic amines (BAs) in Xinjiang smoked horsemeat sausages. The conditions UPLC-MS/MS, extraction solvent and purification were optimized. internal standard used quantitative analysis. Results showed that BAs exhibited good linearity (1-200 μg/L, correlation coefficient > 0.99). limits detection quantification 5 μg/kg 10 μg/kg, respectively. accuracy between 80.4% 111.4%, precision less than 10%. effectively forty tyramine major amine, followed by cadaverine, putrescine spermine. Total content ranged from 26.5 mg/kg to 352.9 mg/kg, contents sausages may pose a potential health risk.